Gene: Cre14.g630000

Overview

Common name:KIR2
Defline:putative ketoacid isomerase-like protein; (1 of 7) PF12680 - SnoaL-like domain (SnoaL_2)
Description:Related to a series of bacterial genes among which is a putative ketosteroid isomerase-related protein of Ralstonia; however, it is impossible to find a similarity with the well-characterized delta5-3-ketosteroid isomerase of Pseudomonas putida or Comamonas testosteroni, so this may be a case of erroneous annotation in Ralstonia that spread into COG; is probably targeted to the thylakoid lumen (based on alignment and RR motif), like the related gene KIR2
Synonyms:g15172.t1,Cre14.g630000.t1.1,KIR2
Chromosome:chromosome 14

Protein Localization

A plasmid for expressing Cre14.g630000 tagged with the fluorescent protein Venus and affinity epitope 3xFLAG is available: pLM005-Cre14.g630000-Venus-3xFLAG

Imaged strain: CSI_LW04F11

Imaging conditions: Tris-Phosphate media, air, 150 uM photons/m2/s, shaking in 96-well plate; imaged after 16 hours.

Classification(s):

chloroplast/homogeneous/not pyrenoid depleted

If you reference these protein localization data in a manuscript, please cite: Wang et al. 2023.

VenusChlorophyllBright fieldMerged

Protein-Protein Interactions

We did not determine any protein-protein interactions for this gene.

Mutant Strains

Insertion Junction Mutant Strain Insertion Junction Feature Confidence (%) Genome Version
CLIP2.001920_1 CLIP2.001920 CDS 80 6.1
CLIP2.004317_1 CLIP2.004317 outside_mRNA 80 6.1
CLIP2.004498_1 CLIP2.004498 CDS 80 6.1
CLIP2.004498_2 CLIP2.004498 CDS 80 6.1
CLIP2.005431_1 CLIP2.005431 CDS 80 6.1
CLIP2.007190_2 CLIP2.007190 intron 63 6.1
LMJ.RY0402.058753_1 LMJ.RY0402.058753 intron 73 5.5
LMJ.RY0402.093811_2 LMJ.RY0402.093811 intron 58 5.5
LMJ.RY0402.147484_1 LMJ.RY0402.147484 3'UTR 73 5.5
LMJ.RY0402.167282_1 LMJ.RY0402.167282 intron 58 5.5
LMJ.RY0402.210871_2 LMJ.RY0402.210871 CDS 73 5.5
LMJ.RY0402.220739_1 LMJ.RY0402.220739 intron 95 5.5
LMJ.RY0402.220739_2 LMJ.RY0402.220739 intron 95 5.5

Gene Annotations

PFAM:PF12680
PANTHER:
KEGG_ec:
KEGG_orthology:
KOG:
Gene ontology terms::
Best arabidopsis TAIR name:
Best arabidopsis TAIR symbol:
Best arabidopsis TAIR defline:
Phytozome Locus Page

Phenotypes

Our phenotype screens did not find statistically significant results for this gene.