| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.147484 |
| Chromosome: | chromosome 14 |
| Location: | 3339613 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630000 | KIR2 | putative ketoacid isomerase-like protein; (1 of 7) PF12680 - SnoaL-like domain (SnoaL_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAATTAATTTGCTCGACGACACAAGGCTG |
| Internal bar code: | CGTGTTTCATATGGCGTTGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 473 |
| LEAP-Seq percent confirming: | 99.2806 |
| LEAP-Seq n confirming: | 276 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAGTCAGTGTGCGTGAGG |
| Suggested primer 2: | GGGAGTGTCGTTGTTGGAGT |