Insertion junction: CLIP2.000068_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.000068
Chromosome:chromosome 12
Location:4932178
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g524300 CGL71,PYG7 (1 of 7) 2.4.1.255 - Protein O-GlcNAc transferase / OGTase; Pale yellow green 7, a PSI assembly factor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTCGGTCTCCGGTCTCCACTGGTAGGTTTTCCCGATTGTCCTCTACAC
Internal bar code:TTTGGCGTAGTGCATTCAGAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1207
LEAP-Seq percent confirming:51.2821
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:CTGAGGGACGGAGAGTGGTA
Suggested primer 2:GTTGCAGGTGTGTAAGTGCG