Insertion junction: CLIP2.000078_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.000078
Chromosome:chromosome 12
Location:3344658
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g498500 DEG1C,DEG11 (1 of 1) PF00089//PF13180 - Trypsin (Trypsin) // PDZ domain (PDZ_2); Deg protease CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCATCGCCGACCCGACCGGCAAGGGCGCATCCTCCGGCGTGGGCTTCGCG
Internal bar code:TAGCGTACGTCTAAGCCTTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:163
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CTAACCTAGGCGAGCTCAGC
Suggested primer 2:TCCAAGCAGTAACCCGTCAC