Insertion junction: CLIP2.000081_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.000081
Chromosome:chromosome 3
Location:1134766
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g148950 CGL43,SRRP1,PNT1 Ortholog of PSII biogenesis protein SRRP1; (1 of 3) IPR003029//IPR012340//IPR022967 - S1 domain // Nucleic acid-binding, OB-fold // RNA-binding domain, S1 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCGCCCTTGTTGCCCCCCCCGTACATATGAACTTGATAAGCACCGCGC
Internal bar code:TGCGGGGAACTGTTATCTACCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1024
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CTTTAGGAATTGCCTCGCGC
Suggested primer 2:AAGTCTGGGTTATGGGCAGC