Insertion junction: CLIP2.000108_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.000108
Chromosome:chromosome 3
Location:5010979
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g179800 LCI24 Low-CO2-inducible membrane protein; (1 of 5) PF07466 - Protein of unknown function (DUF1517) (DUF1517) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTTGCGAGCAGAGAAGCCGCTGGAGCCACCCACGCGGCCACCGGAGCG
Internal bar code:GTGGTTACTCCTTCCTCTCTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1214
LEAP-Seq percent confirming:95.3488
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCGCTGGACTTGTCTTTA
Suggested primer 2:CGGGTGCCAAGTTCAAACAG