| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.000141 |
| Chromosome: | chromosome 3 |
| Location: | 1918893 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g155001 | ISA1,STA7 | (1 of 1) PTHR10357:SF169 - ISOAMYLASE 1, CHLOROPLASTIC; Isoamylase, starch debranching enzyme 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCCCGCCGCCGCCTCCACGGGGTCAGGCTCCAACCGCTCCAACTGGC |
| Internal bar code: | CCTTCAAACGTGTCGTAGGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 343 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTCAGGCTCTAGACACAG |
| Suggested primer 2: | TGTGGAACATGGGTAAGGCC |