| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.000149 |
| Chromosome: | chromosome 13 |
| Location: | 2514595 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g580000 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTTCCACTATTGTGATGGGCGTTGCTGCCCTGGCCTTCTGCACCTGGT |
| Internal bar code: | ATGCAAAAGTTGGTCGTTTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 175 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAAGGCCAAGTTCACAGC |
| Suggested primer 2: | CGTTTACAGCATCCTTCGCG |