| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.000156 |
| Chromosome: | chromosome 16 |
| Location: | 3230759 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g665800 | SS4,SSS4 | Soluble starch synthase IV; (1 of 2) PTHR12526:SF304 - STARCH SYNTHASE 4, CHLOROPLASTIC/AMYLOPLASTIC-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGGGAGATGAGAGAGGACGAGGTTGGCAGAACGCGGTGGTGCCATACA |
| Internal bar code: | TTTAGGAGTCGGAAGTTAGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4042 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAGCCGGGGTCAGTTTGTG |
| Suggested primer 2: | TTGGAGAAAAGGACGAGGGC |