| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.000211 |
| Chromosome: | chromosome 10 |
| Location: | 2717761 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g437800 | (1 of 3) IPR000104//IPR001054//IPR006059//IPR029787 - Antifreeze protein, type I // Adenylyl cyclase class-3/4/guanylyl cyclase // Solute-binding family 1 // Nucleotide cyclase | CDS | |
| Cre10.g437829 | CIA6,CGL89 | (1 of 1) PTHR13271//PTHR13271:SF19 - UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE // PROTEIN SET DOMAIN GROUP 40; methyltransferase domain protein involved in pyrenoid biogenesis | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAGTTTCGGCATTTGAGCACTGTGCCGAGCCTGTTTGACGAGCCCCAGC |
| Internal bar code: | TCCCGCGCACACCTACCAGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3564 |
| LEAP-Seq percent confirming: | 98.2143 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCATGTTTATCGCCAGC |
| Suggested primer 2: | AGGAAGGTTGGGCTGGATTG |