Insertion junction: CLIP2.000347_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.000347
Chromosome:chromosome 10
Location:4936404
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g453900 CYN71 (1 of 1) K12736 - peptidylprolyl isomerase domain and WD repeat-containing protein 1 [EC:5.2.1.8] (PPWD1); Cyclophilin 71 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCATTCCAAACGCAAACATAAACATCCTAATAACATGAATTCATCGCA
Internal bar code:CTACGGGGCATTGGGAGTCAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:971
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGTAGGGAGGGGAGTGCA
Suggested primer 2:ACACACACACACACACAGGT