| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.000361 |
| Chromosome: | chromosome 3 |
| Location: | 2930132 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g162800 | LCI1 | Low-CO2-inducible membrane protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGATGGAGGGGAAGACGGTGCAGGCGGCGGTCAGCAGGAACACAAACA |
| Internal bar code: | AGCCTCGTACGACAGGGACTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1432 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCGGACTGCCCTTATCCT |
| Suggested primer 2: | GCCACTGAACCTAACGACCA |