| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.000422 |
| Chromosome: | chromosome 10 |
| Location: | 3496025 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444700 | SBE3,SBE2b | (1 of 3) 2.4.1.18 - 1,4-alpha-glucan branching enzyme / Glycogen branching enzyme; Starch Branching Enzyme 3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAGCTTCTCTCCCGGAGCAGCAGCGGTCACAACAGAACGCGGTAGCGCG |
| Internal bar code: | GCTCTCTGAAATTTAAACACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2529 |
| LEAP-Seq percent confirming: | 98.4127 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTACTCCACTGCCACGTTG |
| Suggested primer 2: | CCACTCGCGGTACCAGATAC |