| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.000422 |
| Chromosome: | chromosome 10 |
| Location: | 3496032 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444700 | SBE3,SBE2b | (1 of 3) 2.4.1.18 - 1,4-alpha-glucan branching enzyme / Glycogen branching enzyme; Starch Branching Enzyme 3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCTATCGGCTCTGGAGCTACTGCGTCGCGAGAACGAGCTCCTGCGCCA |
| Internal bar code: | CTCTGTAGTGCTCGTCGGTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1098 |
| LEAP-Seq percent confirming: | 95.7747 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACTCGCGGTACCAGATAC |
| Suggested primer 2: | ACTACTCCACTGCCACGTTG |