| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.000455 |
| Chromosome: | chromosome 8 |
| Location: | 1080424 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g362450 | AMYA2,AMA2 | Alpha-amylase 2; (1 of 1) PTHR10357//PTHR10357:SF118 - ALPHA-AMYLASE // ALPHA-AMYLASE 2-RELATED | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAGAGGTCACGTTGGCGCGAATCCTCTTTGAATTGCGTAACGTGCTTG |
| Internal bar code: | CCGCCCAAAGATCGGTAAGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2048 |
| LEAP-Seq percent confirming: | 98.3333 |
| LEAP-Seq n confirming: | 59 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGGAGGGGTGTTGCAAAA |
| Suggested primer 2: | GGACTCTCCACAACTCTGCC |