Insertion junction: CLIP2.000528_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.000528
Chromosome:chromosome 12
Location:9102479
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g544150 CYN20-2,CYN7,TLP2,NSG1,CYN20B (1 of 4) K03768 - peptidyl-prolyl cis-trans isomerase B (cyclophilin B) [EC:5.2.1.8] (PPIB, ppiB); Cyclophilin 20-2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCCCGCCGGCTGGCCGCCCACAGACACGTCGAAGTAAACCTTCTGCGT
Internal bar code:TGTGTCAAATCCTTCGCTACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1388
LEAP-Seq percent confirming:92.5926
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGGAGAAGGGCCTTGGTC
Suggested primer 2:GTGCAGAGTCAGTCCGTGAA