Insertion junction: CLIP2.000590_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.000590
Chromosome:chromosome 12
Location:3470066
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g497300 TEF2,CAS,CAS1 Rhodanese-like Ca-sensing receptor; (1 of 25) PF00581 - Rhodanese-like domain (Rhodanese) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAGATATACACTACCCGAGTTTCTCCGACTCCTTGACGGTTATCTCTCT
Internal bar code:TAGAGAGAGCAATTTAATCTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3594
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:87
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:87

Suggested primers for confirmation by PCR

Suggested primer 1:GAACCCCCAGGACATTGAGG
Suggested primer 2:TGTACCACACGCTGGAGAAC