Gene: Cre12.g497300
Overview
| Common name: | TEF2,CAS,CAS1 |
| Defline: | Rhodanese-like Ca-sensing receptor; (1 of 25) PF00581 - Rhodanese-like domain (Rhodanese) |
| Description: | Present in thylakoid-enriched fraction based on mass spectrometric peptide identification [PMID: 17078018]. CAS is required for the expression of HLA3 and LCIA which are cooperatively associated with bicarbonate uptake for CO2-concentraing mechanism [PMID:24549931, 27791081]; CAS localization displays a mesh-like structure in the chloroplast under high-CO2 conditions, but CAS gathers inside the pyrenoid as a distinct wheel-like structure at 2 h after transfer to low-CO2 condition, and then localized to the |
| Synonyms: | TEF2,CAS,g12639.t2,Cre12.g497300.t1.1 |
| Chromosome: | chromosome 12 |
Protein Localization
A plasmid for expressing Cre12.g497300 tagged with the fluorescent protein Venus and affinity epitope 3xFLAG is available: pLM005-Cre12.g497300-Venus-3xFLAG
Imaged strain: CSI_LW03E5
Imaging conditions: Tris-Phosphate media, air, 150 uM photons/m2/s, shaking in 96-well plate; imaged after 16 hours.
Classification(s):
chloroplast/pyrenoid/pyrenoid traversing enriched
If you reference these protein localization data in a manuscript, please cite: Wang et al. 2023.
 |  |  |  |
| Venus | Chlorophyll | Bright field | Merged |
Protein-Protein Interactions
| Bait ID | Bait Name | Prey ID | Prey Name | Reference |
|---|---|---|---|---|
| Cre01.g050950 | CHLP1,CHLP,LYL1 | Cre12.g497300 | TEF2,CAS,CAS1 | Wang et al. 2023 |
| Cre06.g278195 | CTI,CTI1 | Cre12.g497300 | TEF2,CAS,CAS1 | Wang et al. 2023 |
Mutant Strains
| Insertion Junction | Mutant Strain | Insertion Junction Feature | Confidence (%) | Genome Version |
|---|---|---|---|---|
| CLIP2.000056_1 | CLIP2.000056 | CDS | 80 | 6.1 |
| CLIP2.000056_2 | CLIP2.000056 | CDS | 80 | 6.1 |
| CLIP2.000189_1 | CLIP2.000189 | 3'UTR | 80 | 6.1 |
| CLIP2.000189_2 | CLIP2.000189 | 3'UTR | 80 | 6.1 |
| CLIP2.000590_1 | CLIP2.000590 | 3'UTR | 80 | 6.1 |
| CLIP2.000590_2 | CLIP2.000590 | 3'UTR | 80 | 6.1 |
| CLIP2.000729_1 | CLIP2.000729 | CDS | 80 | 6.1 |
| CLIP2.000757_1 | CLIP2.000757 | CDS | 80 | 6.1 |
| CLIP2.000757_2 | CLIP2.000757 | CDS | 80 | 6.1 |
| LMJ.RY0402.131739_1 | LMJ.RY0402.131739 | intron | 95 | 5.5 |
| LMJ.RY0402.131739_2 | LMJ.RY0402.131739 | intron | 95 | 5.5 |
Gene Annotations
| PFAM: | PF00581 |
| PANTHER: | PTHR34209 |
| KEGG_ec: | |
| KEGG_orthology: | |
| KOG: | |
| Gene ontology terms:: | |
| Best arabidopsis TAIR name: | AT5G23060 |
| Best arabidopsis TAIR symbol: | CaS |
| Best arabidopsis TAIR defline: | calcium sensing receptor |
| Phytozome Locus Page |
Phenotypes
Our phenotype screens did not find statistically significant results for this gene.