Insertion junction: CLIP2.000729_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.000729
Chromosome:chromosome 12
Location:3468506
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g497300 TEF2,CAS,CAS1 Rhodanese-like Ca-sensing receptor; (1 of 25) PF00581 - Rhodanese-like domain (Rhodanese) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCGAGTTCGGCCTGGGCGCCGTGGCGCTGTATTACCTCAGCCCCCTGT
Internal bar code:CGACATCGGGGTAAATGGGATA

Confirmation - LEAP-Seq

LEAP-Seq distance:3236
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCAATGTCCTGGGGGTTC
Suggested primer 2:GGCTTACCTGGCTGTCCATT