| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.131739 |
| Chromosome: | chromosome 12 |
| Location: | 3366141 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g497300 | TEF2,CAS,CAS1 | Rhodanese-like Ca-sensing receptor; (1 of 25) PF00581 - Rhodanese-like domain (Rhodanese) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATCTGATCCGCTGCTCTCACCGAGATGGC |
| Internal bar code: | GTTGGAGTTGGGCGACGATGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 844 |
| LEAP-Seq percent confirming: | 90.6892 |
| LEAP-Seq n confirming: | 4500 |
| LEAP-Seq n nonconfirming: | 462 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGCGTAGCACCACTGTT |
| Suggested primer 2: | CGTCAAGAAGCTTTTCTGGG |