Insertion junction: CLIP2.000633_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.000633
Chromosome:chromosome 13
Location:2289741
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g578650 CPLD10,NUOAF5,HCF173 (1 of 1) PTHR10366//PTHR10366:SF343 - NAD DEPENDENT EPIMERASE/DEHYDRATASE // SUBFAMILY NOT NAMED; PSII biogenesis protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATCATGAATGTAACCCGCCCCCGCCCCCACACAGGCTGACGAGGACAAT
Internal bar code:TGGATGTACATTAAGATTCTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:5171
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:48
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:GTATCGCTCTCCACTGGCTC
Suggested primer 2:ACAACGCGTCTACTCTGCAA