Insertion junction: CLIP2.000661_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.000661
Chromosome:chromosome 3
Location:5010657
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g179800 LCI24 Low-CO2-inducible membrane protein; (1 of 5) PF07466 - Protein of unknown function (DUF1517) (DUF1517) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTTCGACTAGCTCAGCCTACCTTACAACTAAGTGCGCGTACACCAATAC
Internal bar code:ATTGGTGTGTCCGACGACTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2433
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:CGGGTGCCAAGTTCAAACAG
Suggested primer 2:CGTTCTTGTGCTTGTGTGCA