| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.000674 |
| Chromosome: | chromosome 14 |
| Location: | 3776853 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g632000 | CGE2,OPR65 | GrpE nucleotide release factor; (1 of 3) K03687 - molecular chaperone GrpE (GRPE) | 5'UTR |
| Cre14.g632050 | POB25,RPG1,RPGRIP1 | Retinitis pigmentosa GTPase regulator-interacting protein 1; (1 of 1) PF11618 - First C2 domain of RPGR-interacting protein 1 (C2-C2_1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTACGCAAGAAACCGGGCTCGAGAGAAGTCTAGAGCTCGCTCGAATGCC |
| Internal bar code: | ACAAGCGCTGTAGCGGATAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 651 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAGGACCGCAGATGTAGA |
| Suggested primer 2: | CCGACAGGTGAAAGCTTCCT |