| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.000675 |
| Chromosome: | chromosome 12 |
| Location: | 4734467 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g522950 | MIND1 | Chloroplast septum site-determining protein; (1 of 1) K03609 - septum site-determining protein MinD (minD) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAGAACGACATGATGTCAGTGAAGGACGTGCAGGAGATGCTCGGCATC |
| Internal bar code: | GTGAATGCCCTTTTAGAGTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4315 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTGAAAGCAGGTCGTCAT |
| Suggested primer 2: | GCATTGACGTCGGCTTCATC |