| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.000718 |
| Chromosome: | chromosome 12 |
| Location: | 8296064 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551200 | (1 of 5) 3.2.1.1 - Alpha-amylase / Glycogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGTGAAGCAGGGCCAGCCGACGTCGATTAGCGCCCAGCCGCGTGCTCT |
| Internal bar code: | GATGTGTTCGCCAGTCACACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 768 |
| LEAP-Seq percent confirming: | 5.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGTACTGCATCGGGTAGG |
| Suggested primer 2: | AGCAGCGTCCAGTCGATATG |