Insertion junction: CLIP2.000728_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.000728
Chromosome:chromosome 6
Location:5988172
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g290400 HDA5 (1 of 1) PTHR10625:SF104 - HISTONE DEACETYLASE 6; RPD3/HDA1 type histone deacetylase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAGGAACTCCTCTTCACCCAGGTCCCCCAGGTCACTCTCACCCGCACC
Internal bar code:GTCAGGTTGAGCTTCGGACGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1381
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CTACTGGACCTCCTCGACCA
Suggested primer 2:TCAGCCTTTTTGTGCATGGC