| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.000811 |
| Chromosome: | chromosome 10 |
| Location: | 2720773 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g437829 | CIA6,CGL89 | (1 of 1) PTHR13271//PTHR13271:SF19 - UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE // PROTEIN SET DOMAIN GROUP 40; methyltransferase domain protein involved in pyrenoid biogenesis | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAATTGGAAGTACTGAGCAAGTTTTGGCGCCATAGAAGAAGTTGGCGCG |
| Internal bar code: | GCGCGGGGCTAGTGTGCTTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3472 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGTGCTGTGCGGGAATTT |
| Suggested primer 2: | AAGATTGGCACTACGCGTGA |