Insertion junction: CLIP2.000819_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.000819
Chromosome:chromosome 10
Location:4937130
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g453900 CYN71 (1 of 1) K12736 - peptidylprolyl isomerase domain and WD repeat-containing protein 1 [EC:5.2.1.8] (PPWD1); Cyclophilin 71 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAATCATGCCTGCGGAAGACGGTTTTCGGGTTCGGGTTTCGGGGTTTGG
Internal bar code:ATGGGAACAGGCTATCGCACCC

Confirmation - LEAP-Seq

LEAP-Seq distance:120
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CACCACCCATAGACACCCAC
Suggested primer 2:AAAGGCCCCCAGACAAAACA