| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.000824 |
| Chromosome: | chromosome 1 |
| Location: | 4203516 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g028350 | DEG8 | (1 of 2) PTHR22939:SF99 - PROTEASE DO-LIKE 8, CHLOROPLASTIC; Deg protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGCCAGCGCAGCGCATCGTTAGGGCACCCGTTTCATGCCGCGTAGCCC |
| Internal bar code: | GAAATCGGGTTTATTGTTGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 537 |
| LEAP-Seq percent confirming: | 78.9474 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGATACCGACAGAAGGGCG |
| Suggested primer 2: | TATTCCCTATGCCGCTGCTG |