| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.000874 |
| Chromosome: | chromosome 16 |
| Location: | 2811487 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g662600 | BST1,LCI11,LCI11C | (1 of 10) PF01062 - Bestrophin, RFP-TM, chloride channel (Bestrophin); Bestrophin 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTAACGCTGCACAAACAATTCACTCACGCTCAGGTTTCAGTCACTGGAT |
| Internal bar code: | AAACATCCCGCGTTGTGTTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1739 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCATCAGCCTCATCCCCT |
| Suggested primer 2: | AAGGGCAGAACGGTTAGTGG |