Insertion junction: CLIP2.000889_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.000889
Chromosome:chromosome 3
Location:5012190
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g179800 LCI24 Low-CO2-inducible membrane protein; (1 of 5) PF07466 - Protein of unknown function (DUF1517) (DUF1517) 3'UTR
Cre03.g179820 3'UTR
lncRNA_TCONS_00067107 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGACGAGTCTGCAATCACCACTGTTGTGCGTCCCGGACTTCTTGATCTCC
Internal bar code:AGGCTCACTTGGGGACCAGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1791
LEAP-Seq percent confirming:91.4286
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTTTGAACTTGGCACCCG
Suggested primer 2:TTCCATGACGTTCACGGAGG