Insertion junction: CLIP2.001014_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.001014
Chromosome:chromosome 17
Location:3097922
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g720450 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTATGACCATTGAACCGCGAACGCGTGTCCGTGTTATTGTGCCCGCTGCG
Internal bar code:ACGGCGGGTCGTGAAACGCATA

Confirmation - LEAP-Seq

LEAP-Seq distance:2744
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TTGCGTTCTGAATCGTCCGA
Suggested primer 2:GTTAGGAGGAAGGGCCCAAC