| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.001167 |
| Chromosome: | chromosome 1 |
| Location: | 3849513 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025250 | RFK2 | Riboflavin kinase; (1 of 1) PTHR22749//PTHR22749:SF2 - RIBOFLAVIN KINASE/FMN ADENYLYLTRANSFERASE // RIBOFLAVIN KINASE | 5'UTR |
| Cre01.g025300 | RAD51B | (1 of 1) K10869 - RAD51-like protein 1 (RAD51L1, RAD51B); DNA recombination protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTTGGTTTTGCATGTTTAAAGGCTCTCAGCATGCAGTGTTGTTGCTAA |
| Internal bar code: | TACTATTAAACGCAGACTGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1011 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGAGATGACTCCACCGGCA |
| Suggested primer 2: | CTGTGACCGAGTAGAGGGGA |