Insertion junction: CLIP2.001232_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.001232
Chromosome:chromosome 12
Location:4142833
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g517350 LSD1 (1 of 1) PTHR31747//PTHR31747:SF1 - FAMILY NOT NAMED // PROTEIN LOL1; zinc-finger protein LSD1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCGTCAATGGGTAGGGTCGCATACGACACTTTCTGTCTGTCGGTTTCC
Internal bar code:GTAATTTTCCATCTAGCTGCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3985
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:71
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:71

Suggested primers for confirmation by PCR

Suggested primer 1:AACAGCCCAGGTCCATTCAG
Suggested primer 2:GCGCGATTAGCTTCACACTG