| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.001233 |
| Chromosome: | chromosome 12 |
| Location: | 1185072 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g490500 | LCAA1,YCF54,CGL78 | (1 of 1) PF10674 - Protein of unknown function (DUF2488) (Ycf54); Magnesium protoporphyrin IX monomethyl ester oxidative cyclase, YCF54 subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCAATGAGGATGGCCACGTAGGGGCTTGCACGCCGTGCGCGTGCCTTC |
| Internal bar code: | GGACATGTGCATGTTTCTGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3891 |
| LEAP-Seq percent confirming: | 75.3086 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 81 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCTCACTGCGTCACACAC |
| Suggested primer 2: | TCTTCGATGCCGCTGTTTCT |