Insertion junction: CLIP2.001258_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.001258
Chromosome:chromosome 11
Location:2115450
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467747 (1 of 2) PF10173 - Mitochondrial K+-H+ exchange-related (Mit_KHE1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTTCCTTAACTAATCATGAATGTACCCCCACCCCCTTACACCGCACAA
Internal bar code:GATACTATAGGGGAGTGCGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:641
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CATCCCAAACCTCCCTTCCC
Suggested primer 2:AAACCAACGAAAACGAGCGG