Insertion junction: CLIP2.001320_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.001320
Chromosome:chromosome 13
Location:2934919
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g583550 VIP1,VIPP1 (1 of 2) K03969 - phage shock protein A (pspA); Vesicle inducing protein in plastids 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGTTAGGCCCACTGTGCCCGACCTGCCAGGTTGTCTGCCCCCCCCTCCC
Internal bar code:CTAGTCTATGGCGTGATAAGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1431
LEAP-Seq percent confirming:90.9091
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCCAACCTGACTGACACC
Suggested primer 2:ACGCAAAAATGTCGCTGCTT