Insertion junction: CLIP2.001440_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.001440
Chromosome:chromosome 1
Location:7488191
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g052750 (1 of 1) PF06011//PF13418 - Transient receptor potential (TRP) ion channel (TRP) // Galactose oxidase, central domain (Kelch_4); Transient receptor potential ion channel intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATACTATTGCATGGCAAAACAACGCATTTTCTGAGAGCGATGAGGCATCC
Internal bar code:CTGATCGAGGACCTACCTCCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:477
LEAP-Seq percent confirming:53.8462
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:ACTGGAGGGTACGCAACTTG
Suggested primer 2:GTAGATACCCTTGTCCGCCG