Insertion junction: CLIP2.001541_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.001541
Chromosome:chromosome 12
Location:9608855
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g539650 (1 of 1) PF00530//PF08016 - Scavenger receptor cysteine-rich domain (SRCR) // Polycystin cation channel (PKD_channel) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGACCTCCACGCGCCACTCGCCAGCAGCAGAACGGCACAGGTGTCGTTGC
Internal bar code:GGTCTATCGTGCCTCTAAGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2047
LEAP-Seq percent confirming:48.1481
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:42
LEAP-Seq n unique pos:81

Suggested primers for confirmation by PCR

Suggested primer 1:TTACTTGCTGTCGCTACGCA
Suggested primer 2:GGGGTTGGACAGGTGTATGG