Insertion junction: CLIP2.001543_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.001543
Chromosome:chromosome 12
Location:6031671
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g534650 (1 of 66) 2.7.12.1 - Dual-specificity kinase 5'UTR
Cre12.g534700 FKB19 (1 of 1) PTHR10516:SF179 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP19, CHLOROPLASTIC; peptidyl-prolyl cis-trans isomerase, FKBP-type gene_edge/outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTACTGTCAACTGGCAAAGCTGCGCTTGAATTCGCGATGCAACCTCCTC
Internal bar code:TGAGTAGCGTGGCATTTTCTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2665
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTTTGGTGGGACCCTTAT
Suggested primer 2:GCCACAGTGGTAACTTCGGA