| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.001550 |
| Chromosome: | chromosome 1 |
| Location: | 7007530 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g050100 | DPD1 | Diaminopimelate decarboxylase; (1 of 1) K01586 - diaminopimelate decarboxylase (lysA) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTTTCAGCGCCCGCCAACCACAGAACGCTAAGTTGCATGCAGATAAAT |
| Internal bar code: | AACGTTACTGGTATTGGTTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 906 |
| LEAP-Seq percent confirming: | 43.75 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGAAATCCACGTCAGCCT |
| Suggested primer 2: | TCGTCCTGTGCTGTGGTAAC |