| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.001559 |
| Chromosome: | chromosome 12 |
| Location: | 4358254 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g519300 | TEF9 | (1 of 2) PF13301 - Protein of unknown function (DUF4079) (DUF4079); conserved expressed protein, possible chloroplast localization | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCCGCTACTGGCCCCTTGCGCCCCACATCGCCACATCAGCAACCACGC |
| Internal bar code: | GCAGGAAATCGACAGCTCTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1015 |
| LEAP-Seq percent confirming: | 64.2857 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAGACACACACGTCCACG |
| Suggested primer 2: | TCCATACATACGCTCGGCAC |