Insertion junction: CLIP2.001665_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.001665
Chromosome:chromosome 7
Location:4634484
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g344550 (1 of 3) 1.1.1.95 - Phosphoglycerate dehydrogenase / Phosphoglyceric acid dehydrogenase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATACCGAACATGTTACTAGGTGCCATCACTCCACCTCCTCTTCCATTAT
Internal bar code:GAAAGGAATATAAGTGGCCGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:3425
LEAP-Seq percent confirming:96.1538
LEAP-Seq n confirming:50
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:52

Suggested primers for confirmation by PCR

Suggested primer 1:CTGACCTGGGGGAAGTTGAC
Suggested primer 2:ACCTGCTCATGCTGCTTCTT