| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.001713 |
| Chromosome: | chromosome 1 |
| Location: | 6833774 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g049000 | PTD1,RAF2,CGL31 | (1 of 1) PTHR12599//PTHR12599:SF8 - PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE // SUBFAMILY NOT NAMED; RuBisCO assembly chaperone, chloroplastic | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGCGTCAGCGTCAGGCTCTCGCTGTGGCCCTCATCCGAGGCCACCTT |
| Internal bar code: | GTTGGCGTTGTTTAAGGTGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3196 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGGTCAGCATAGCATTG |
| Suggested primer 2: | CACATCGCGGCCCTATTACT |