| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.001721 |
| Chromosome: | chromosome 10 |
| Location: | 5327204 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g456750 | DAR1 | (1 of 1) 1.8.5.1 - Glutathione dehydrogenase (ascorbate); dehydroascorbate reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAACGCATAGCGAGTCCCTGCTCAAGCTATTGCGCACTGCCAGCAGTC |
| Internal bar code: | GTCACCCTGACATGATACCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4003 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 72 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGGGACCCTCCTTGATCA |
| Suggested primer 2: | TTGTGCTCTGGATGGTGGAC |