Gene: Cre10.g456750

Overview

Common name:DAR1
Defline:(1 of 1) 1.8.5.1 - Glutathione dehydrogenase (ascorbate); dehydroascorbate reductase
Description:Catalyzes the reduction of dehydroascorbate to ascorbate in environmental stress adaptation. Similar to Arabidopsis At1g1957.1
Synonyms:DAR1,Cre10.g456750.t1.1,g11208.t1
Chromosome:chromosome 10

Protein Localization

A plasmid for expressing Cre10.g456750 tagged with the fluorescent protein Venus and affinity epitope 3xFLAG is available: pLM005-Cre10.g456750-Venus-3xFLAG

Imaged strain: CSI_LW08H9

Imaging conditions: Tris-Phosphate media, air, 150 uM photons/m2/s, shaking in 96-well plate; imaged after 16 hours.

Classification(s):

chloroplast/homogeneous/pyrenoid depleted

cytosol/cytosol (strong signal)

nucleus/nucleoplasm

nucleus/nucleolus

(with enriched signal in nucleoplasm)

If you reference these protein localization data in a manuscript, please cite: Wang et al. 2023.

VenusChlorophyllBright fieldMerged

Protein-Protein Interactions

We did not determine any protein-protein interactions for this gene.

Mutant Strains

Insertion Junction Mutant Strain Insertion Junction Feature Confidence (%) Genome Version
CLIP2.001721_1 CLIP2.001721 intron 80 6.1
CLIP2.001721_2 CLIP2.001721 intron 80 6.1
CLIP2.001790_1 CLIP2.001790 intron 80 6.1
CLIP2.001790_2 CLIP2.001790 intron 80 6.1
CLIP2.004102_1 CLIP2.004102 intron 80 6.1
CLIP2.004102_2 CLIP2.004102 intron 80 6.1
CLIP2.005907_1 CLIP2.005907 CDS 80 6.1
CLIP2.005907_2 CLIP2.005907 CDS 80 6.1
CLIP2.007428_1 CLIP2.007428 intron 80 6.1
CLIP2.007428_2 CLIP2.007428 intron 80 6.1
LMJ.RY0402.041454_1 LMJ.RY0402.041454 intron 95 5.5
LMJ.RY0402.041454_2 LMJ.RY0402.041454 intron 95 5.5
LMJ.RY0402.054746_1 LMJ.RY0402.054746 intron 73 5.5
LMJ.RY0402.061959_1 LMJ.RY0402.061959 3'UTR 58 5.5
LMJ.RY0402.061959_2 LMJ.RY0402.061959 3'UTR 73 5.5
LMJ.RY0402.150701_3 LMJ.RY0402.150701 CDS 58 5.5
LMJ.RY0402.150701_4 LMJ.RY0402.150701 3'UTR 73 5.5
LMJ.RY0402.180186_2 LMJ.RY0402.180186 intron 58 5.5
LMJ.RY0402.206114_1 LMJ.RY0402.206114 3'UTR 73 5.5
LMJ.SG0182.010226_1 LMJ.SG0182.010226 intron 75 5.3

Gene Annotations

PFAM:PF13417, PF16865
PANTHER: PTHR11260 PTHR11260:SF216
KEGG_ec:EC:1.8.5.1
KEGG_orthology:
KOG:KOG1422
Gene ontology terms::GO:0005515
Best arabidopsis TAIR name:AT1G75270
Best arabidopsis TAIR symbol:DHAR2
Best arabidopsis TAIR defline:dehydroascorbate reductase 2
Phytozome Locus Page

Phenotypes

Mutants in this gene exhibit a phenotype under the following conditions:

Photosynthesis or light sensitivity, screen 6 of 12 ( 86% confidence (FDR = 0.14))
Photosynthesis or light sensitivity, screen 7 of 12 ( 84% confidence (FDR = 0.16))

Note: FDR indicates the likelihood that a particular genotype-phenotype relationship is incorrect. Not all mutants in a particular gene show the same phenotype. Please see the mutant pages for each mutant's phenotypes. Complementation is needed to definitively prove a genotype-phenotype link. These data are from a pooled phenotyping experiment.

If you reference the phenotypic data above in a manuscript, please cite: Fauser et al. 2022 Nature Genetics.