| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.180186 |
| Chromosome: | chromosome 10 |
| Location: | 5181353 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g456750 | DAR1 | (1 of 1) 1.8.5.1 - Glutathione dehydrogenase (ascorbate); dehydroascorbate reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCATATGCTTATCTTTAAGCCTGCGGCT |
| Internal bar code: | CCCGACGGTAGTGAGGGGGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 74 |
| LEAP-Seq percent confirming: | 2.24719 |
| LEAP-Seq n confirming: | 98 |
| LEAP-Seq n nonconfirming: | 4263 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGTTGAGCATCCATCCGT |
| Suggested primer 2: | GTACCTGCGGTGGAAACATT |