| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.150701 |
| Chromosome: | chromosome 10 |
| Location: | 5183056 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g456750 | DAR1 | (1 of 1) 1.8.5.1 - Glutathione dehydrogenase (ascorbate); dehydroascorbate reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTGGATTCAGGAAGGCGAACCAAGGAT |
| Internal bar code: | CTCGGCCATTTACTAGGGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 195 |
| LEAP-Seq percent confirming: | 99.5624 |
| LEAP-Seq n confirming: | 455 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGAAGGGTGGGTGAACAG |
| Suggested primer 2: | ACAGCATTGCGACATAGTGC |