Insertion junction: CLIP2.001745_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.001745
Chromosome:chromosome 12
Location:3103841
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g500950 CLPP4 (1 of 3) PTHR10381//PTHR10381:SF24 - ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT // SUBFAMILY NOT NAMED; active subunit of the chloroplast ClpP complex intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTTCGCTGCTGCCAGCTGCGGGCTGGCACGTCAGGTGACCCACCGCGCC
Internal bar code:TGATGAAATGCCCCTAGTAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1789
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTCCTAGACTCCCAGGAC
Suggested primer 2:GGTTGTGAAGTAGGGGAGGC