Insertion junction: CLIP2.001747_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.001747
Chromosome:chromosome 3
Location:2153353
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g156900 LHCBM5 (1 of 6) K08912 - light-harvesting complex II chlorophyll a/b binding protein 1 (LHCB1); Light-harvesting Chlorophyll a/b binding protein of LHCII intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCGGAGCGCGCGCTTCCCCGCCGCTTCTACCCGCCCGCATGTGTTAGCA
Internal bar code:TGCGGCGCGGCCAGCACTCTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:268
LEAP-Seq percent confirming:44.4444
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GCAAATCACGCACCGTTCTT
Suggested primer 2:ACTCATGCGCTAGGAGATGC